Hippocampal Protein Expression Analysis

Western blot analysis was performed as described previously [3 (link),6 (link)]. Hippocampal tissues were homogenized with lysis buffer. Protein content was measured using a Bio-Rad colorimetric protein assay kit (Bio-Rad, Hercules, CA, USA). The protein mixture containing 40 μg total protein was separated on sodium dodecyl sulfate-polyacrylamide gels and transferred onto a nitrocellulose membrane. Mouse actin antibody (1:2,000; Santa Cruz Biotechnology), mouse Bax antibody (1:1,000; Santa Cruz Biotechnology) and mouse Bcl-2 antibody (1:1,000; Santa Cruz Biotechnology) were used as primary antibodies. Horseradish peroxidase-conjugated antimouse antibodies for Bax and Bcl-2 (1:2,000; Amersham Pharmacia Biothech GmbH, Freiburg, Germany) were used as secondary antibodies. Bands were detected using an enhanced chemiluminescence detection kit (Santa Cruz Biotechnology).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Park J.H., Kim S.E., Jin J.J., Choi H.S., Kim C.J, & Ko I.G. (2016). Pentoxifylline Alleviates Perinatal Hypoxic-Ischemia-Induced Short-term Memory Impairment by Suppressing Apoptosis in the Hippocampus of Rat Pups. International Neurourology Journal, 20(2), 107-113.

Publication 2016

Bio-Rad colorimetric protein assay kit Mouse actin antibody mouse Bax antibody mouse Bcl-2 antibody enhanced chemiluminescence detection kit

Actin Antibodies Antibody Assay Bcl 2 Buffer Chemiluminescence Colorimetric Horseradish peroxidase Membrane Mouse Nitrocellulose Polyacrylamide gels Protein Sodium dodecyl sulfate Tissues Western blot analysis

Corresponding Organization :

Other organizations : International St. Mary's Hospital, Catholic Kwandong University, Kyung Hee University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of actin, Bax, and Bcl-2

control variables

Hippocampal tissue samples
Lysis buffer
Bio-Rad colorimetric protein assay kit
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Nitrocellulose membrane
Mouse actin, Bax, and Bcl-2 primary antibodies
Horseradish peroxidase-conjugated anti-mouse secondary antibodies
Enhanced chemiluminescence detection kit

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!