Generation and Use of Genetically Modified Mice

C57BL/6/J and LysM-Cre (B6.129p2-Lyz2tm1(cre)lfo/J) mice were purchased from Jackson Laboratory and housed in the animal facility of University of Lausanne. Macrophage-specific Cas9 knock-in were generated as previously described^{29 (link)}. Macrophage-specific XBP1 Knockout mice were generated by crossing LysM-Cre mice (myeloid-specific overexpression of Cre recombinase) with XBP1^fl/fl mice. Macrophages-specific Lpcat3 knockout mice were generated by crossing LysM-Cre mice with Lpcat3^fl/fl mice^{53 (link)}. BRafCA; Tyr::CreER; Ptenlox4-5 (Braf/PTEN) was obtained from M. Bonsenburg at Yale University. Bone marrow of Mincle-knockout mice was provided by David Sancho^{54 (link)}. All experiments were performed in accordance with Swiss federal regulations and procedures ethically approved by veterinary authority of Canton Vaud.

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Di Conza G., Tsai C.H., Gallart-Ayala H., Yu Y.R., Franco F., Zaffalon L., Xie X., Li X., Xiao Z., Raines L.N., Falquet M., Jalil A., Locasale J.W., Percipalle P., Masson D., Huang S.C., Martinon F., Ivanisevic J, & Ho P.C. (2021). Tumor-induced reshuffling of lipid composition on the ER membrane sustains macrophage survival and pro-tumorigenic activity. Nature immunology, 22(11), 1403-1415.

Publication 2021

C57BL/6/J LysM-Cre (B6.129p2-Lyz2tm1(cre)lfo/J)

Animal Bone marrow Braf Cre recombinase Knockout mice Macrophages Mice Pten Xbp1

Corresponding Organization :

Other organizations : University of Lausanne, New York University Abu Dhabi, Ludwig Cancer Research, Duke University, University School, Case Western Reserve University, Université de Bourgogne, Inserm

Top 5 similar protocols

Variable analysis

independent variables

LysM-Cre (B6.129p2-Lyz2tm1(cre)lfo/J) mice
Macrophage-specific Cas9 knock-in mice
Macrophage-specific XBP1 Knockout mice
Macrophages-specific Lpcat3 knockout mice
BRafCA; Tyr::CreER; Ptenlox4-5 (Braf/PTEN) mice
Mincle-knockout mice

dependent variables

Not explicitly mentioned

control variables

C57BL/6/J mice

controls

Positive control: C57BL/6/J mice
Negative control: Not specified

Annotations

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