Acquired LC-MS and GC-MS data were processed using the Thermo Scientific TraceFinder 5.0 software. Targeted metabolites were identified by matching one target and at least one confirming ion and retention time (GC-MS) or accurate mass and retention times (LC-MS) to the University of Iowa Metabolomics Core Facility’s in-house library of confirmed standards. After peak area integration by TraceFinder, NOREVA software was applied for signal drift correction on a metabolite-to-metabolite basis using the pooled QC sample that had been analyzed throughout the instrument run (16 (link)). Within individual samples, Post-NOREVA metabolite levels were divided by the total metabolite load (sum of all metabolite values) to equally weight individual metabolites for comparison across samples.
Protocol detail
Targeted Metabolomics Data Processing
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Gc ms
Library
Retention
Corresponding Organization : Fraternal Order of Eagles
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- Metabolite levels
- Pooled QC sample analyzed throughout the instrument run
- Positive control: Pooled QC sample
- Negative control: Not mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!