Generation of Viral Expression Constructs

pcDNA-3xFLAG-Vpr and pscAAV-mCherry-T2A-Vpr WT and mutant plasmids were generated as previously described (28 (link)). For rAAV production, pHelper and pAAV-2.5 capsid plasmids were used (Addgene and (28 (link)). For VLP production, psPAX2 and pmD2.G were used (Addgene). For HIV-1 ΔENV production, Bru-GFP ΔENV was generated as previously described (62 (link)) and pmD2.G (Addgene). For lentivirus stable knock-down of DCAF1, pLKO.1 (Addgene), psPAX2(Addgene) and pmD2.G (Addgene) were used.

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Sandoval C, & Fregoso O.I. (2023). HIV-1 Vpr-induced DNA damage activates NF-κB independent of cell cycle arrest and CRL4ADCAF1 engagement. bioRxiv.

Publication Preprint 2023

psPAX2 pmD2.G pLKO.1

Capsid Dcaf1 Hiv 1 Knock Lentivirus Plasmids

Corresponding Organization : University of California, Los Angeles

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Variable analysis

independent variables

PcDNA-3xFLAG-Vpr and pscAAV-mCherry-T2A-Vpr WT and mutant plasmids
PHelper and pAAV-2.5 capsid plasmids
PsPAX2 and pmD2.G
Bru-GFP ΔENV
PLKO.1, psPAX2, and pmD2.G

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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