Apoptosis and Autophagy Detection Methods

Apoptosis was assayed by Muse^® Annexin V & Dead Cell Assay (MCH100105, Merck Millipore, Darmstadt, Germany). The apoptosis detections of blood smears were achieved by Apoptag Plus Fluorscein in situ Apoptosis Detection Kit (S7111, Millipore, Temecula, USA) according to the instruction. Rapamysin (RAPA) and 3-MA purchased from SelleckChem (San Diego, CA) were utilized to enhance or recede autophagy level according to the previous papers39 (link)66 (link).

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Liu Y.W., Yang T., Zhao L., Ni Z., Yang N., He F, & Dai S.S. (2016). Activation of Adenosine 2A receptor inhibits neutrophil apoptosis in an autophagy-dependent manner in mice with systemic inflammatory response syndrome. Scientific Reports, 6, 33614.

Publication 2016

Muse® Annexin V & Dead Cell Assay

Annexin v Apoptosis Assay Autophagy Blood Cell Muse

Corresponding Organization :

Other organizations : Army Medical University, Daping Hospital

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Variable analysis

independent variables

Rapamysin (RAPA)

dependent variables

Apoptosis
Autophagy level

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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