Epoxy Sample Imaging Protocol

Images of the epoxy sections were obtained with a microscope (Nikon 90i) equipped with a Nikon DS digital camera. For each section, four partly overlapping digital images were recorded and combined into one 24‐bit colour image using analySIS (version 3.2, Soft Imaging System).
All images were aligned by a custom‐made computer program running on Linux (openSUSE 11.2, www.opensuse.org) as previously described in detail (Chang et al., 2015 (link)). The final image size was 2700 × 2000 pixels with an isotropic pixel size of 0.93 μm.

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Cong J., Chang S., Thomsen J.S., Andreasen A., Chen X., Xing J., Zhang J., Gu L, & Zhai X. (2021). Ultrastructural identification of developing proximal tubules based on three‐dimensional reconstruction. Veterinary Medicine and Science, 7(5), 1989-1998.

Publication 2021

analySIS (version 3.2,

Epoxy Microscope

Corresponding Organization : China Medical University

Other organizations : Shenyang Medical College, Aarhus University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

Microscope (Nikon 90i) equipped with a Nikon DS digital camera
Analytic software (analySIS version 3.2)
Custom-made computer program running on Linux (openSUSE 11.2)
Final image size (2700 × 2000 pixels)
Isotropic pixel size (0.93 μm)

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

Annotations

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