RNA was prepared as described (Noble et al, 2008 (link)). 0.5 μg RNA was reverse transcribed using Superscript III (Invitrogen) according to the manufacturer’s instructions, and 1 μl of the product was subjected to PCR using ReddyMix™ (Thermo Scientific). Quantitative RT–PCR was performed as described (Noble et al, 2008 (link)). Primers used are described in Supplementary Methods.
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