The U2OS, U2OS Baf180 KO and Baf180sh, and U2OS reporter cell lines (U2OS 263 IFII and U2OS 265, Tang et al., 2013 (link)) were maintained in a 5% CO2 incubator at 37°C in GIBCO DMEM media (Life Technologies, Paisley, UK) supplemented with 100 U/mL penicillin/streptomycin, 2 mM L-glutamine and 10% FCS (U2OS) or 10% TET System approved FCS (U2OS reporter cell lines; 631106, Takara Bio).
LNCaP cells were obtained from ATCC (Clone FGC, CRL-1740) and cultured in GIBCO RPMI media (Life Technologies, Paisley, UK) supplemented with 10% FCS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, 10 mM HEPES and 1 mM sodium pyruvate. All cell lines were regularly tested for mycoplasma contamination.
LNCaP cells were obtained from ATCC (Clone FGC, CRL-1740) and cultured in GIBCO RPMI media (Life Technologies, Paisley, UK) supplemented with 10% FCS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, 10 mM HEPES and 1 mM sodium pyruvate. All cell lines were regularly tested for mycoplasma contamination.
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