vimentin overexpressed plasmid was purchased from Addgene 31922: pvimentin-PSmOrange with full length of vimentin sequence insertion [56 (link)]. Plasmids of vimentin knockdown were purchased from National RNAi Core Facility Platform in Taiwan:

shLuc: TRCN0000072244, ATCACAGAATCGTCGTATGCA,

shVim #3: TRCN0000029119, GCTAACTACCAAGACACTATT,

shVim #4: TRCN0000029121, GCAGGATGAGATTCAGAATAT.

Cells were transfected by different shVim sequences in a cell population and then selected by Puromycin for two weeks to generate stable clones for further experiment. vimentin overexpressing stable clones were transfected in a cell population and then selected by Neomycin, followed by sorting to purify the vimentin expressing cells.
siRNA were purchased from Thermo (L-003551-00-0005, ON-TARGETplus SMARTpool, Human Vim) combined four siRNA sequences together:

J-003551-06, UCACGAUGACCUUGAAUAA;

J-003551-07, GAGGGAAACUAAUCUGGAU;

J-003551-08, UUAAGACGGUUGAAACUAG;

J-003551-09, GGAAAUGGCUCGUCACCUU.

And the control scramble siRNA sequence was (D-001810-01-05, ON-TARGET plus Non-targeting siRNA #1): UGGUUUACAUGUCGACUAA
The primary antibodies used in the study, were as followed: β1-integrin (BD), GAPDH (Santa Cruz), vimentin (Millipore), vinculin (SIGMA), paxillin (BD), E-cadherin (BD), β-catenin (BD), slug (Cell Signaling), filamin A (Cell Signaling), and p-filamin A (Cell Signaling).
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