Western Blot Analysis of Cellular Proteins

Western blot analyses were performed as described previously and repeated twice.^{14 (link),32 (link)} Primary antibodies used in this study include rabbit polyclonal antibodies to human NS (Ab138, Tsai Laboratory, Houston, TX, USA), p-cdc2 (Y15, Cell Signaling, Danvers, MA, USA), MDM2 (SMP-14, Santa Cruz, Santa Cruz, CA, USA), p53 (DO-1, Santa Cruz), p-histone H3 (S10, Upstate, Lake Placid, NY, USA), and α-tubulin (Sigma). Secondary antibodies were conjugated to peroxidase (Jackson ImmunoResearch, West Grove, PA, USA).

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Huang G., Meng L, & Tsai R. (2015). p53 Configures the G2/M arrest response of nucleostemin-deficient cells. Cell Death Discovery, 1, 15060-.

Publication 2015

p-cdc2 (Y15, MDM2 (SMP-14, p53 (DO-1, α-tubulin peroxidase

Antibodies Cdc2 Histone h3 Human Mdm2 Peroxidase Rabbit Western blot analyses α tubulin

Corresponding Organization :

Other organizations : Texas A&M University, Texas A&M Health Science Center

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Variable analysis

independent variables

Rabbit polyclonal antibodies to human NS (Ab138, Tsai Laboratory, Houston, TX, USA)
Rabbit polyclonal antibodies to p-cdc2 (Y15, Cell Signaling, Danvers, MA, USA)
Rabbit polyclonal antibodies to MDM2 (SMP-14, Santa Cruz, Santa Cruz, CA, USA)
Rabbit polyclonal antibodies to p53 (DO-1, Santa Cruz)
Rabbit polyclonal antibodies to p-histone H3 (S10, Upstate, Lake Placid, NY, USA)
Mouse monoclonal antibodies to α-tubulin (Sigma)

dependent variables

Western blot analyses

control variables

Western blot analyses were performed as described previously and repeated twice

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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