Cells were transfected with equal concentration of the dual GFP–ChFP fluorescence reporter containing K20 internal repeat [poly(A)] or K0 control (Sundaramoorthy et al., 2017 (link)) in 293T cells. Fluorescence levels were measured using an X-20 Fortessa (BD) instrument, as described previously (Sundaramoorthy et al., 2017 (link)).
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