Antibody Preparation and Characterization

Rabbit anti-l-afadin and rabbit anti-l/s-afadin were prepared as described [13] (link). The Abs listed below were purchased from commercial sources: rat anti-nectin-1, clone 48–12 (MBL); rat anti-nectin-3, clone 103-A1 (MBL); mouse anti-N-cadherin, clone 32 (BD Biosciences); rabbit anti-N-cadherin (Takara); rabbit anti-β-catenin (Sigma); rabbit anti-synapsin I (Millipore); guinea pig anti-VGLUT1 (Millipore); mouse anti-bassoon (Enzo Life Sciences); mouse anti-PSD-95, clone 7E3-1B8 (Enzo Life Sciences) and clone K28/43 (NeuroMab); mouse anti-actin (clone C4) (Millipore); and chicken anti-MAP2 (Abcam). Alexa Fluor-conjugated secondary Abs (Life Technologies) were used for immunohistochemistry and immunocytochemistry.

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Toyoshima D., Mandai K., Maruo T., Supriyanto I., Togashi H., Inoue T., Mori M, & Takai Y. (2014). Afadin Regulates Puncta Adherentia Junction Formation and Presynaptic Differentiation in Hippocampal Neurons. PLoS ONE, 9(2), e89763.

Publication 2014

mouse anti-N-cadherin, clone 32 rabbit anti-β-catenin rabbit anti-synapsin I guinea pig anti-VGLUT1 mouse anti-bassoon mouse anti-actin (clone C4) chicken anti-MAP2

Actin Afadin Cadherin Chicken Clone Guinea pig Immunocytochemistry Immunohistochemistry Map2 Mouse Nectin 1 Nectin 3 Rabbit Synapsin i β catenin

Corresponding Organization : Hyogo University of Health Sciences

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Variable analysis

independent variables

Rabbit anti-l-afadin
Rabbit anti-l/s-afadin

dependent variables

Expression/localization of nectin-1
Expression/localization of nectin-3
Expression/localization of N-cadherin
Expression/localization of β-catenin
Expression/localization of synapsin I
Expression/localization of VGLUT1
Expression/localization of bassoon
Expression/localization of PSD-95
Expression/localization of actin
Expression/localization of MAP2

control variables

Rat anti-nectin-1, clone 48-12 (MBL)
Rat anti-nectin-3, clone 103-A1 (MBL)
Mouse anti-N-cadherin, clone 32 (BD Biosciences)
Rabbit anti-N-cadherin (Takara)
Rabbit anti-β-catenin (Sigma)
Rabbit anti-synapsin I (Millipore)
Guinea pig anti-VGLUT1 (Millipore)
Mouse anti-bassoon (Enzo Life Sciences)
Mouse anti-PSD-95, clone 7E3-1B8 (Enzo Life Sciences) and clone K28/43 (NeuroMab)
Mouse anti-actin, clone C4 (Millipore)
Chicken anti-MAP2 (Abcam)
Alexa Fluor-conjugated secondary Abs (Life Technologies)

Annotations

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