HPLC Analysis of δ-Toxin

HPLC analysis was performed using the previously described method [28 (link)] or a new method established in this study. Chromatography was performed using a Kromasil 100-5-C18 (AkzoNobel) by Agilent 1100 series. Water/acetonitrile gradient in 0.1% trifluoroacetic acid from 50% to 100% acetonitrile for 30 min at a flow rate of 1 mL/min. Absorbance at 215 nm was detected using a photodiode array detector. The respective δ-toxin peaks were confirmed using peptide and MALDI/TOF-MS (see below).

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Zhou X., Zheng Y., Lv Q., Kong D., Ji B., Han X., Zhou D., Sun Z., Zhu L., Liu P., Jiang H, & Jiang Y. (2021). Staphylococcus aureus N-terminus formylated δ-toxin tends to form amyloid fibrils, while the deformylated δ-toxin tends to form functional oligomer complexes. Virulence, 12(1), 1418-1437.

Publication 2021

Kromasil 100-5-C18 Agilent 1100 series

Acetonitrile Chromatography Hplc Maldi ms Peptide Toxin Trifluoroacetic acid

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University

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Variable analysis

independent variables

Gradient of water/acetonitrile from 50% to 100% acetonitrile

dependent variables

Absorbance at 215 nm

control variables

Chromatography performed using a Kromasil 100-5-C18 (AkzoNobel) by Agilent 1100 series
Flow rate of 1 mL/min
Gradient duration of 30 min

controls

Peptide and MALDI/TOF-MS analysis to confirm the respective δ-toxin peaks

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