HeLa cells were grown on glass coverslips and RNA was microinjected using InjectMan coupled with FemtoJet (Eppendorf) as described previously30 (link),95 . For microinjection of denatured U2WT snRNA, RNA was incubated at 98 °C for 5 min and immediately microinjected into the HeLa cells. After 1 h incubation period, cells were rinsed twice with PBS and fixed for 20 min at room temperature in 4% PFA/PIPES (freshly prepared).
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