Oral Glucose Tolerance in Diabetic Rats

As shown in Figure 1, after 3-week treatment in diet-STZ-induced diabetic rats, an oral glucose tolerance test (OGTT) was performed. After a 12 h fast, all the experimental rats were received physiological saline, metformin, AE, or WE, respectively, as described above; 30 min later, 2 g/kg of glucose was orally given to all the rats. Blood samples were collected at 0, 30, 60, and 120 min to detect the blood glucose levels using Glucose Assay Kit (Nanjing Biotechnology Co. Ltd., Jiangsu, China). Calculation of the area under the blood glucose curve (AUC) was made according to ((I)) [25 (link)]:
$\begin{array}{cc}\left(\text{I}\right)& \text{AUC}=(\text{basal}\hspace{0.17em}\hspace{0.17em}\text{glycaemia}+\text{glycaemia}\hspace{0.17em}\hspace{0.17em}\mathrm{0.5}\hspace{0.17em}\text{h})\times \mathrm{0.25}\\ +(\text{glycaemia}\hspace{0.17em}\hspace{0.17em}\mathrm{0.5}\hspace{0.17em}\text{h}+\text{glycaemia}\hspace{0.17em}\hspace{0.17em}\mathrm{1}\hspace{0.17em}\text{h})\times \mathrm{0.25}\\ +(\text{glycaemia}\hspace{0.17em}\hspace{0.17em}\mathrm{1}\hspace{0.17em}\text{h}+\text{glycaemia}\hspace{0.17em}\hspace{0.17em}\mathrm{2}\hspace{0.17em}\text{h})\times \mathrm{0.5}.\end{array}$