Whole-Body Composition Measurement by DXA

Whole body composition was measured at year 22 using a Hologic QDR 4500A DXA machine and analysed using Apex software version 4.0.2 (Hologic Inc., Bedford, USA). All scans were performed according to standard procedures by a trained technician. The machine was calibrated daily using a phantom spine, and coefficients of variation during the course of the study were <2% for total fat mass, and 1% for fat-free soft tissue mass. All standard DXA measurements were analysed using Hologic APEX 3.1 software (Hologic). Whole body (excluding head) fat free soft tissue mass (FFSTM) and fat mass were recorded. Abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were measured according to previously described methodology [22 (link)].

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Prioreschi A., Munthali R.J., Kagura J., Said-Mohamed R., De Lucia Rolfe E., Micklesfield L.K, & Norris S.A. (2018). The associations between adult body composition and abdominal adiposity outcomes, and relative weight gain and linear growth from birth to age 22 in the Birth to Twenty Plus cohort, South Africa. PLoS ONE, 13(1), e0190483.

Publication 2018

QDR 4500A DXA machine Apex software version 4.0.2 Hologic APEX 3.1 software

Abdominal adipose tissue Body Body composition Head Scans Spine Subcutaneous adipose tissue

Corresponding Organization : University of the Witwatersrand

Other organizations : MRC Epidemiology Unit, University of Cambridge

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Whole body (excluding head) fat free soft tissue mass (FFSTM)
Whole body (excluding head) fat mass
Abdominal visceral adipose tissue (VAT)
Abdominal subcutaneous adipose tissue (SAT)

control variables

Whole body composition measured at year 22 using a Hologic QDR 4500A DXA machine
DXA scans performed according to standard procedures by a trained technician
Machine calibrated daily using a phantom spine
Coefficients of variation during the course of the study were <2% for total fat mass, and 1% for fat-free soft tissue mass
All standard DXA measurements analyzed using Hologic APEX 3.1 software (Hologic)

controls

Positive control: Phantom spine used for daily calibration of the DXA machine
Negative control: Not explicitly mentioned

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