A list of all the strains used and produced in this study is provided in S1 Table . Aspergillus nidulans strain IBT 29539 (argB2, pyrG89, veA1, nkuAΔ)—referred to as NID1—was used for heterologous production of niduclavin and niduporthin. A. clavatus genomic DNA was obtained from strain IBT 12364 (NRRL 1) and was extracted using the FastDNATM SPIN Kit for Soil DNA extraction (MP Biomedicals, USA). Coding sequence of M. oryzae genes syn2 and rap2 were purchased from GenScript USA. Escherichia coli strain DH5α was used for plasmid propagation.
Aspergillus solid and liquid minimal medium (MM) and transformation medium (TM) was supplemented when necessary and according to strain genotypes with 4 mM L-arginine, 10 mM uridine, 10 mM uracil, and 1.3 mg/ml 5-fluoroorotic acid (5-FOA), and was prepared as described by Nødvig et al. [44 (link)]. E. coli DH5α was cultivated in Luria-Bertani (LB) medium consisting of 10 g/l tryptone (Bacto), 5 g/l yeast extract (Bacto), and 10 g/l NaCl (pH 7.0). LB medium was supplemented with 100 µg/ml ampicillin. All solvent used was of HPLC grade, and H2O was purified and deionized by a Millipore system through a 0.22 µm membrane filter (MQ H2O).
Aspergillus solid and liquid minimal medium (MM) and transformation medium (TM) was supplemented when necessary and according to strain genotypes with 4 mM L-arginine, 10 mM uridine, 10 mM uracil, and 1.3 mg/ml 5-fluoroorotic acid (5-FOA), and was prepared as described by Nødvig et al. [44 (link)]. E. coli DH5α was cultivated in Luria-Bertani (LB) medium consisting of 10 g/l tryptone (Bacto), 5 g/l yeast extract (Bacto), and 10 g/l NaCl (pH 7.0). LB medium was supplemented with 100 µg/ml ampicillin. All solvent used was of HPLC grade, and H2O was purified and deionized by a Millipore system through a 0.22 µm membrane filter (MQ H2O).
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