Neural recording data fed as input to feedforward and recurrent networks were acquired from rat cortical neurons (A1084001, lot 2214638, Gibco Thermo Fisher Scientific) cultured on microelectrode arrays (MEAs; 60MEA100/10iR-ITO-gr, Harvard Biosciences) with methods described previously (25 (link), 56 (link)). MEAs were coated with 0.1 mg/mL polyethyleneimine (408727, Sigma-Aldrich) and 4 μg/mL laminin (23017-015, Thermo Fisher Scientific). A volume of 50 µL of cells was plated for 4 h at 1 million cells/mL on sterilized MEA in media (Neurobasal Plus + 1× GlutaMAX + 10% fetal bovine serum; Thermo Fisher), maintained in media without serum, and replaced every 2–3 days. Data were recorded between days in vitro (DIV) 18 and DIV 22 with an MZ60 MEA headstage amplifier at 6,104 Hz per channel streamed through PZ5 neurodigitizer amplifier and RZ5P base processor (Tucker-Davis Technologies, Alachua, FL). Total recording duration for each data set was 900 s, beginning with a 60-s stimulation epoch. Stimulation was delivered globally by ambient temperature change (from 37°C to room temperature, 60 s). Raw data files were loaded into MATLAB with TDT Synapse software environment and development kit for processing and analysis.
Free full text: Click here
