Sources of cell lines are as previously described (21 (link)). All cell lines have been maintained in a central bank at Moffitt Cancer Center since 2008, authenticated by STR analysis (ACTG Inc, Wheeling, IL) as of September 2010, and are routinely tested and negative for mycoplasma (PlasmoTest, InvivoGen, San Diego, CA). Experiments were conducted <15 passages post thaw. Viability assays were conducted with Cell Titer Glo assay kits (Promega) and read on Spectramax M5 plate reader. For siRNA-mediated knockdown, On-Target Smartpools (Dharmacon) were used and transfections performed with Lipofectamine RNAiMax (Invitrogen).
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Cell Line Authentication and Viability Assays
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Corresponding Organization : Moffitt Cancer Center
Other organizations : University of Colorado Denver, Montefiore Medical Center, Albert Einstein College of Medicine, Cornell University
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Variable analysis
independent variables
- SiRNA-mediated knockdown
- Cell viability
- Cell lines maintained in a central bank at Moffitt Cancer Center since 2008
- Cell lines authenticated by STR analysis as of September 2010
- Cell lines routinely tested and negative for mycoplasma
- Experiments conducted within 15 passages post thaw
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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