A drop of Flura-Safe Fluorexon Disodium & Benoxinate Hydrochloride Ophthalmic Solution 0.35%/0.4% (Apollo Ophthalmics, Newport Beach, CA, USA) was placed over the mouse eyeballs for 1 minute, and the excess removed with PBS washes. The eyeballs were imaged using a Zeiss Discovery.V12 Stereo Microscope (Zeiss). At least seven eyeballs were used for each experimental point.
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Fluorexon and Benoxinate Ophthalmic Imaging
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Corresponding Organization : University of Houston
Other organizations : Universidade Federal de São Paulo, Sanford Burnham Prebys Medical Discovery Institute, Linkou Chang Gung Memorial Hospital, Chang Gung University, Cleveland Clinic
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Variable analysis
independent variables
- Application of Flura-Safe Fluorexon Disodium & Benoxinate Hydrochloride Ophthalmic Solution 0.35%/0.4% on mouse eyeballs
- Appearance of mouse eyeballs imaged using a Zeiss Discovery.V12 Stereo Microscope
- Duration of Flura-Safe Fluorexon Disodium & Benoxinate Hydrochloride Ophthalmic Solution 0.35%/0.4% application (1 minute)
- Removal of excess solution with PBS washes
- Number of eyeballs used for each experimental point (at least seven)
- No positive or negative controls were explicitly mentioned in the provided information.
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