Synthesis and Characterization of CPTEG:SA Copolymer

A 10:90 CPTEG:SA copolymer was synthesized as described previously [41 (link)]. Briefly, the diacids were acetylated at 140 °C for 30 min, and then reacted at 180 °C at 0.2 torr for 30–60 min, dissolved into methylene chloride (Thermo Fisher Scientific, Waltham, MA, USA), precipitated into hexanes (Thermo Fisher Scientific), and dried. The molecular weight of the copolymer was ~15,000 g/mol, as confirmed by proton nuclear magnetic resonance (NMR; MR-400 MHz, Varian, Palo Alto, CA, USA) spectroscopy.

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Schlichtmann B.W., Palanisamy B.N., Malovic E., Nethi S.K., Padhi P., Hepker M., Wurtz J., John M., Ban B., Anantharam V., Kanthasamy A.G., Narasimhan B, & Mallapragada S.K. (2023). Aggregation-Inhibiting scFv-Based Therapies Protect Mice against AAV1/2-Induced A53T-α-Synuclein Overexpression. Biomolecules, 13(8), 1203.

Publication 2023

methylene chloride hexanes MR-400 MHz

Hexanes Methylene chloride Proton Sa copolymer Spectroscopy

Corresponding Organization : Iowa State University

Other organizations : PathoVacs (United States), Indiana Biosciences Research Institute, University of Georgia

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Variable analysis

independent variables

Synthesis temperature (140 °C)
Synthesis duration (30-60 min)

dependent variables

Molecular weight of the copolymer (~15,000 g/mol)

control variables

Diacid acetylation
Reaction pressure (0.2 torr)
Solvent (methylene chloride)
Precipitant (hexanes)

controls

No positive or negative controls were explicitly mentioned.

Annotations

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