A 290 mm long × 21 mm wide LH-20 column was selected as the stationary phase to separate 300 mg of dried B. concava extract dissolved in 3 mL of methanol/ethyl acetate (1:1). To elute the column, methanol/ethyl acetate (1:1) was used as mobile phase. A total of 40 fractions of approximately 5 mL each were collected, resolved by TLC, and pooled by their patterns, as shown in Figure 2 . Finally, antimicrobial activity was determined to continue with further chemical characterization [50 (link)].
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