Identifying Mitochondrial Genome Homology

All newly identified transcripts were subjected to an NCBI-BLASTN homology search (v. 2.13.0; Altschul et al., 1990 (link); default parameters, except word size = 7) against the Plasmodium falciparum mitochondrial genome (M76611.1) and the Eimeria leuckarti mitochondrial genome (MW354691.1). Transcripts not matching the P. falciparum or E. leuckarti mitochondrial genome were further inspected for homology to the E. coli rRNA operon (J01695.2) using BLASTN.

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Tetzlaff S., Hillebrand A., Drakoulis N., Gluhic Z., Maschmann S., Lyko P., Wicke S, & Schmitz-Linneweber C. (2024). Small RNAs from mitochondrial genome recombination sites are incorporated into T. gondii mitoribosomes. eLife, 13, e95407.

Publication 2024

Corresponding Organization :

Other organizations : Humboldt-Universität zu Berlin

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Variable analysis

independent variables

NCBI-BLASTN homology search parameters (e.g., word size = 7)

dependent variables

Transcripts matching the Plasmodium falciparum or Eimeria leuckarti mitochondrial genome
Transcripts not matching the Plasmodium falciparum or Eimeria leuckarti mitochondrial genome but showing homology to the Escherichia coli rRNA operon

control variables

Plasmodium falciparum mitochondrial genome (M76611.1)
Eimeria leuckarti mitochondrial genome (MW354691.1)
Escherichia coli rRNA operon (J01695.2)

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