All crystals were glued to cantilever (Arrow TL- 1, Nanoworld) with epoxy (21 (link)) at least 12 h before use. All attraction forces between cell and crystal were measured with JPK CellHesion (JPK) (23 (link)). Briefly, the measurement was carried in the relative force feedback contact/tapping mode (IP gain: 50 Hz; IG gain: 0.001 Hz; correct baseline: 1; relative set point: 0.5 nN; z length: 50 µm; extend delay: 0 s; constant height). Data were analyzed with JPK Data Processing. Maximum binding forces were calculated and plotted. Each dot presents a single measurement.
Attraction forces between crystal and lipid monolayer were measured with JPK NanoWizard II (JPK). All set up was the same as the JPK CellHesion except z length was 10 µm. AFM scanning mode was used to confirm domain formation.
Attraction forces between crystal and lipid monolayer were measured with JPK NanoWizard II (JPK). All set up was the same as the JPK CellHesion except z length was 10 µm. AFM scanning mode was used to confirm domain formation.
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