Characterizing T Cell Responses to Viral Infection

WT (CD4-Cre, ERT2-Cre-LSL-YFP), CD4-Cre Panx1^fl/fl, ERT2-Cre-LSL-YFP-Panx1^fl/fl, and CD4-Cre P2rx7^fl/fl P14 cells were adoptively transferred into naive wild-type mice, which were infected with LCMV-Armstrong (2 × 10⁵ PFU, intraperitoneally (i.p.)). Sometimes, wild-type (CD4-Cre, CMV-Cre), CMV-Cre Panx1^fl/fl or CD4-Cre Panx1^fl/fl mice were infected with LCMV Armstrong. In other experiments, wild-type (CD4-Cre) or CD4-Cre Panx1^fl/fl mice were infected with Influenza-PR8 (1400 PFU, intranasally (i.n.)). In some experiments, LCMV-infected mice were treated with Trovafloxacin (4.2 mg x kg⁻¹ mouse, Sigma-Aldrich) or Sodium Lactate (1.68 g x kg⁻¹ mouse, Sigma-Aldrich)^{72 (link)} between days 1–7 after infection.