BAM15 was dissolved in saline with 10% ethanol and 10% Cremophor EL at a concentration of 1 mg/ml. BAM15 was then injected intraperitoneally into nine BALB/c female mice at a dose of 10 mg/kg. Blood samples (100 μl) were collected at 0 h, 0.083 h, 0.25 h, 0.5 h, 1 h, 2 h, 4 h, 8 h, 12 h and 24 h. After all blood samples had been centrifuged at 3000 rpm for 10 min, plasma samples were collected and immediately stored at − 20 °C until analysis. Then, BAM15 levels in mouse plasma were determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).
Pharmacokinetic parameters were calculated using WinNonlin professional software version 8.0 (Pharsight, Mountain View, CA, USA). The optimal pharmacokinetic model was determined according to the principle of the minimum Akaike information criterion (AIC) value and used for data fitting and parameter estimation. The area under the plasma curve (AUC), peak plasma concentration (Cmax), half-life (T1/2) and peak time (Tmax) are expressed as the mean ± SD [20 (link)].
Pharmacokinetic parameters were calculated using WinNonlin professional software version 8.0 (Pharsight, Mountain View, CA, USA). The optimal pharmacokinetic model was determined according to the principle of the minimum Akaike information criterion (AIC) value and used for data fitting and parameter estimation. The area under the plasma curve (AUC), peak plasma concentration (Cmax), half-life (T1/2) and peak time (Tmax) are expressed as the mean ± SD [20 (link)].
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