Serum aspartate transaminase (AST) and alanine transferase (ALT) levels were determined using Spectrum® (Abbott Laboratories, Abbott Park, IL, USA). Liver tissue samples were fixed using 10% neutral-buffered formalin solution (Sigma, St. Louis, MO, USA), embedded in paraffin blocks, and stained for histological assessment. Masson’s trichrome stained liver sections were assessed by a liver pathologist in a blind manner. Fibrosis extent was graded as 0, absent; 1, enlarged, fibrotic portal areas; 2, periportal or portal-portal septa but intact architecture; 3, fibrosis with architectural distortion but no obvious cirrhosis; and 4, probable or definite cirrhosis.
Protocol detail
Liver Histopathology and Enzyme Levels
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Corresponding Organization :
Other organizations : Seoul National University, Hanyang University, Daegu-Gyeongbuk Medical Innovation Foundation, Chosun University
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Variable analysis
independent variables
- Not explicitly mentioned
- Serum aspartate transaminase (AST) levels
- Serum alanine transferase (ALT) levels
- Fibrosis extent graded as 0, 1, 2, 3, or 4
- The use of Spectrum® (Abbott Laboratories, Abbott Park, IL, USA) to determine AST and ALT levels
- The use of 10% neutral-buffered formalin solution (Sigma, St. Louis, MO, USA) to fix the liver tissue samples
- The embedding of liver tissue samples in paraffin blocks
- The blinded assessment of Masson's trichrome stained liver sections by a liver pathologist
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