Single-Molecule Fluorescence Imaging Setup
Corresponding Organization :
Other organizations : University of Applied Sciences Upper Austria, Johannes Kepler University of Linz
Variable analysis
- XYZ piezo stage for sample positioning
- Illumination through 60× magnification objective lens
- 642 nm diode laser for sample illumination
- Fluorescence signal from immobilized fluorophore–biomolecule conjugates
- Dichroic filter
- Emission filters
- EMCCD camera for fluorescence detection
- 20 ms illumination time
- 4.15 kW cm^-2 excitation intensity at λ_exc = 640 nm
- No positive or negative controls were explicitly mentioned in the input text.
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