Characterization of Il-27 Knockout Mice

All animal studies were approved by the Duke University Institutional Animal Care and Use Committee under protocols A175-14-07, A156-17-06, and A107-20-05. C57BL6/J (The Jackson Laboratory, Stock #000664, Bar Harbor, ME), Il-27p28fl/fl;LysMCre^+/^-, Il-27p28^EGFP mice and their littermates or Il-27p28fl/fl mice were used for control. Il-27p28^EGFP mice were kindly generated (14 (link)) and provided by Dr. Ross M. Kedl. Il-27p28fl/fl mice (33 (link)) were kindly provided by Drs. Zhinan Yin (Biomedical Translational Research Institute, Jinan University) and Li Fan Lu (University of California San Diego). These mice were bred with LysMCre mice (The Jackson Laboratory, Stock #004781) to generate Il-27p28fl/fl;LysMCre^+/^- in our laboratory. Mice were maintained under regulated conditions with food and water ad libitum in the pathogenic-free facility at Duke University.

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Suwanpradid J., Lee M.J., Hoang P., Kwock J., Floyd L.P., Smith J.S., Yin Z., Atwater A.R., Rajagopal S., Kedl R.M., Corcoran D.L., Zhang J.Y, & MacLeod A.S. (2021). IL-27 Derived From Macrophages Facilitates IL-15 Production and T Cell Maintenance Following Allergic Hypersensitivity Responses. Frontiers in Immunology, 12, 713304.

Publication 2021

C57BL6/J LysMCre LysMCre mice

Animal Food Institutional animal care and use committee Mice Pathogenic

Corresponding Organization : Duke University

Other organizations : Jinan University, Zhuhai People's Hospital, University of Colorado Anschutz Medical Campus

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Variable analysis

independent variables

Genotype: C57BL6/J, Il-27p28fl/fl;LysMCre+/-, Il-27p28EGFP, and Il-27p28fl/fl mice

dependent variables

Not explicitly mentioned

control variables

Mice were maintained under regulated conditions with food and water ad libitum in the pathogenic-free facility at Duke University

controls

Positive control: Il-27p28fl/fl mice
Negative control: Littermates of Il-27p28fl/fl;LysMCre+/- mice

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