About 200 mg of powdered cookies were extracted with 80% methanol according to methodology described by Przygodzka et al. [26 (link)]. Then extracted samples were stored in −80 °C before prior analysis of antioxidant and reducing capacity (DPPH, PCL, and FRAP assays).
Therefore, DPPH assay was established to measure antioxidants (extracted from cookies) scavenging ability against DPPH radicals [39 (link)]. The measurement were performed at a microplate reader (Tecan M1000 Infinite PRO) with the wavelength established at 517 nm. Results were presented as mmol Trolox per gram of sample. PCL method was used to measure ability of antioxidants from cookies to scavenge superoxide anion radicals (O2−•). The measurement was performed using PHOTOCHEM apparatus (Analytik Jena, Jena, Germany) according to protocols elaborated by Zieliński et al. [40 (link)]. The extracts of cookies were determined in two methodologies: for lipophilic antioxidants (PCL ACL) and hydrophilic (PCL ACW) methodology extracts of were expressed as µmol Trolox per gram of sample. The reducing power was determined using FRAP assay according to Horszwald and Andlauer [39 (link)]. The mixture’s absorbance was measured at 593 nm after 5 min reaction with microplate reader (M1000 Infinite PRO, Tecan, Männedorf, Switzerland). The FRAP method is based on the reduction of ferric ion by antioxidant compounds.
Therefore, DPPH assay was established to measure antioxidants (extracted from cookies) scavenging ability against DPPH radicals [39 (link)]. The measurement were performed at a microplate reader (Tecan M1000 Infinite PRO) with the wavelength established at 517 nm. Results were presented as mmol Trolox per gram of sample. PCL method was used to measure ability of antioxidants from cookies to scavenge superoxide anion radicals (O2−•). The measurement was performed using PHOTOCHEM apparatus (Analytik Jena, Jena, Germany) according to protocols elaborated by Zieliński et al. [40 (link)]. The extracts of cookies were determined in two methodologies: for lipophilic antioxidants (PCL ACL) and hydrophilic (PCL ACW) methodology extracts of were expressed as µmol Trolox per gram of sample. The reducing power was determined using FRAP assay according to Horszwald and Andlauer [39 (link)]. The mixture’s absorbance was measured at 593 nm after 5 min reaction with microplate reader (M1000 Infinite PRO, Tecan, Männedorf, Switzerland). The FRAP method is based on the reduction of ferric ion by antioxidant compounds.
Free full text: Click here
