SARS-CoV-1 and SARS-CoV-2 Antibody Phagocytic Assay
ADCP was measured as previously described (75 (link)). Briefly, biotinylated SARS-CoV-1 or SARS-CoV-2 prefusion-stabilized S trimer was incubated with red streptavidin-fluorescent beads (Molecular Probes) for 2 h at 37 °C. Ten microliters of a 100-fold dilution of beads–protein was incubated for 2 h at 37 °C with 100 μL of 8,100-fold (SARS-CoV-2) or 900-fold (SARS-CoV-1) diluted plasma samples before addition of THP-1 cells (20,000 cells per well; Millipore Sigma). After 19 h incubation at 37 °C, the cells were fixed with 2% formaldehyde solution (Tousimis), and fluorescence was evaluated on an LSRII flow cytometer (BD Bioscience). The phagocytic score was calculated by multiplying the percentage of bead-positive cells by the geometric mean of the fluorescence intensity of bead-positive cells and dividing by 10,000.
King H.A., Joyce M.G., Lakhal-Naouar I., Ahmed A., Cincotta C.M., Subra C., Peachman K.K., Hack H.R., Chen R.E., Thomas P.V., Chen W.H., Sankhala R.S., Hajduczki A., Martinez E.J., Peterson C.E., Chang W.C., Choe M., Smith C., Headley J.A., Elyard H.A., Cook A., Anderson A., Wuertz K.M., Dong M., Swafford I., Case J.B., Currier J.R., Lal K.G., Amare M.F., Dussupt V., Molnar S., Daye S.P., Zeng X., Barkei E.K., Alfson K., Staples H.M., Carrion R., Krebs S.J., Paquin-Proulx D., Karasavvas N., Polonis V.R., Jagodzinski L.L., Vasan S., Scott P.T., Huang Y., Nair M.S., Ho D.D., de Val N., Diamond M.S., Lewis M.G., Rao M., Matyas G.R., Gromowski G.D., Peel S.A., Michael N.L., Modjarrad K, & Bolton D.L. (2021). Efficacy and breadth of adjuvanted SARS-CoV-2 receptor-binding domain nanoparticle vaccine in macaques. Proceedings of the National Academy of Sciences of the United States of America, 118(38), e2106433118.
Other organizations :
Walter Reed Army Institute of Research, Henry M. Jackson Foundation, Washington University in St. Louis, National Cancer Institute, Center for Cancer Research, United States Army Medical Research Institute of Infectious Diseases, Texas Biomedical Research Institute, Aaron Diamond AIDS Research Center, Columbia University
Dependent Variables not detected. Unable to provide accurate variables.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
