Immunoblotting for ABCB1, ABCG2, and HDAC6

An immunoblot assay using antibodies C219 (#517310, Merck Millipore, Burlington, Massachusetts, USA) at 1:3000, BXP-21 (#ab3380, Abcam, Cambridge, MA, USA) at 1:15,000, anti-HDAC6 (#7558, Cell Signaling Technology, Danvers, MA, USA) at 1:1000, and anti-α-tubulin (#T6199, Sigma-Aldrich, St. Louis, MO, USA) at 1:100,000 was performed to identify ABCB1, ABCG2, HDAC6, and tubulin as a positive control for Western blotting, as described previously [56 (link)]. The horseradish peroxidase-conjugated goat anti-mouse immunoglobulin G (IgG) and anti-rabbit IgG were used as secondary antibodies, and signals were detected as described previously [53 (link)].