IKAROS (GenBank accession no. NM_006060) plasmids used in this study were previously described (Kuehn et al., 2016 (link)). Human AIOLOS (IKZF3) open reading frame clone (pcDNA3.1/C-(K)DYK-IKZF3; NCBI accession no. NM_012481) was purchased from GenScript and subcloned into the mammalian expression vector pcDNA3-HA. Indicated mutants for IKZF3 were generated based on the site-directed mutagenesis protocol using AccuPrime Pfx DNA Polymerase, followed by DpnI treatment (Life Technologies).
Kuehn H.S., Chang J., Yamashita M., Niemela J.E., Zou C., Okuyama K., Harada J., Stoddard J.L., Nunes-Santos C.J., Boast B., Baxter R.M., Hsieh E.W., Garofalo M., Fleisher T.A., Morio T., Taniuchi I., Dutmer C.M, & Rosenzweig S.D. (2021). T and B cell abnormalities, pneumocystis pneumonia, and chronic lymphocytic leukemia associated with an AIOLOS defect in patients. The Journal of Experimental Medicine, 218(12), e20211118.
Clone Dna polymerase Human MammalianPlasmids Site directed mutagenesis Vector
Corresponding Organization : National Institutes of Health Clinical Center
Other organizations :
RIKEN Center for Integrative Medical Sciences, Tokyo Medical and Dental University, University of Colorado Denver, Children's Hospital Colorado
Human AIOLOS (IKZF3) open reading frame clone (pcDNA3.1/C-(K)DYK-IKZF3; NCBI accession no. NM_012481)
Indicated mutants for IKZF3
dependent variables
Not explicitly mentioned
control variables
Not explicitly mentioned
controls
Positive controls: Not specified
Negative controls: Not specified
Annotations
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