Muscle Transcriptome Response to Exercise

RNA was extracted from pulverized muscle biopsy samples at baseline (pre‐exercise) and 3.5‐h after exercise (post‐exercise) using the Qiagen RNeasy mRNA extraction kit and sent to the Mayo Clinic Genome Analysis Core for sequencing as previously described (Kunz et al., 2019 (link); Lalia et al., 2017 (link)). Briefly, libraries were prepared using the Illumina Stranded mRNA Prep and sequenced on an Illumina HiSeq 4000. Reads were aligned using STAR RNA‐seq aligner. Differential expression analysis was carried out using a negative binomial generalized log‐linear model in the edgeR R package. Gene set enrichment analysis was performed using Broads GSEA software. All gene sets that were negatively enriched with an FDR corrected p‐value (using the Benjamini‐Hochberg procedure) < 0.05 were reported.