Spleen and kidney sections were H&E-stained for pathological assessment of tissue architecture and glomerulonephritis. Sections were dried for 30 min at RT and fixed for 15 min in ice-cold acetone. Sections were then rinsed for 2 min in PBS, stained for 2 min with hematoxylin quick stain (Vector Laboratories, Burlingame, CA), and rinsed again in water until clear. Sections were then stained for 1 min with eosin (Vector), rinsed 2 × 5 min in 95% ethanol, rinsed 2 × 5 min in 100%, and cleared for 10 min in a xylene mixture. Slides were mounted with VectaMount medium (Vector Laboratories). Kidney images were deidentified and sent to a pathologist for disease scoring of glomerulonephritis and interstitial nephritis (77 (link)). Scoring was performed on a 0 to 3 scale (0 = absent, 1 = mild, 2 = severe) based on glomerular size, hypercellularity, and sclerosis; interstitial disease was assessed on the basis of the degree of inflammatory infiltrate and alteration in tissue architecture.