Sprague-Dawley rats (Envigo Harlan, Houston, TX, USA) were used as the islet donor. Islet isolation was done using standard collagenase digestion and gradient purification5 (link). After isolation, the islets were cultured in CMRL-1066 (Mediatech, Manassas, VA, USA) supplemented with 10% newborn calf serum (Equitech Bio, Kerrville, TX, USA) and 1% penicillin/streptomycin (Fisher Scientific, Hampton, NH, USA) in a 37°C 5% CO2 incubator.
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Rat Islet Isolation and Culture
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Corresponding Organization : University of California, Irvine
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Variable analysis
independent variables
- Islet isolation method (standard collagenase digestion and gradient purification)
- Islet yield and viability after isolation
- Sprague-Dawley rats as islet donors
- Culturing islets in CMRL-1066 medium supplemented with 10% newborn calf serum and 1% penicillin/streptomycin
- Incubating islets at 37°C in a 5% CO2 environment
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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