The murine model of infantile hemangioma was produced, as described previously,5 (link) with the addition of endothelial progenitor cells isolated from human umbilical-cord blood7 (link) as follows: 1×106 hemangioma-derived stem cells and 7×105 cord-blood endothelial progenitor cells per mouse were mixed, sedimented, resuspended in Matrigel (BD Biosciences), and injected subcutaneously into the backs of 6- to 8-week-old male athymic nu/nu mice (Massachusetts General Hospital). When cord-blood endothelial progenitor cells were included with hemangioma-derived stem cells in the Matrigel implant, the formation of microvessels was enhanced. However, cord-blood endothelial progenitor cells that were implanted alone did not form vessels in this model.8 (link) Microvessel density was quantified as described previously.8 (link) Values that are reported for each experimental condition correspond to the average values obtained from all the individual mice.
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Murine Model of Infantile Hemangioma
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Cord blood
Each
Endothelial progenitor cells
Hemangioma
Human
Infantile hemangioma
Male
Matrigel
Mice
Microvessels
Nu nu
Stem cells
Umbilical cord
Vessels
Corresponding Organization :
Other organizations : Boston Children's Hospital, Harvard University
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Variable analysis
independent variables
- Addition of endothelial progenitor cells isolated from human umbilical-cord blood to the murine model of infantile hemangioma
- Formation of microvessels
- Microvessel density
- Murine model of infantile hemangioma (without the addition of cord-blood endothelial progenitor cells)
- Cord-blood endothelial progenitor cells implanted alone (without hemangioma-derived stem cells)
- Murine model of infantile hemangioma with the addition of endothelial progenitor cells isolated from human umbilical-cord blood
- Murine model of infantile hemangioma (without the addition of cord-blood endothelial progenitor cells)
- Cord-blood endothelial progenitor cells implanted alone (without hemangioma-derived stem cells)
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