LC-MS Analysis of Compounds

For LC–MS analyses, extracts were dissolved in MeOH to a final concentration of 1 mg/mL, and 1 μL was injected into Waters Acquity UPLC system coupled to Agilent 6530 QTOF MS. Samples were analyzed according to the protocol that was previously published^{29 (link)}. LC–MS/MS acquisition of the pure compounds was performed using Shimadzu Nexera X2 UHPLC system coupled to Shimadzu 9030 QTOF mass spectrometer as previously described^{61 (link)}. LC–MS/MS acquisition for molecular networking was performed using Thermo Instruments MS system (LTQ Orbitrap XL, Bremen, Germany) equipped with an electrospray ionization source (ESI) as described^{29 (link)}.

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Machushynets N.V., Elsayed S.S., Du C., Siegler M.A., de la Cruz M., Genilloud O., Hankemeier T, & van Wezel G.P. (2022). Discovery of actinomycin L, a new member of the actinomycin family of antibiotics. Scientific Reports, 12, 2813.

Publication 2022

Acquity UPLC system 6530 QTOF MS Nexera X2 UHPLC system 9030 QTOF mass spectrometer

Ms ms

Corresponding Organization :

Other organizations : Leiden University, Johns Hopkins University, Centre for Human Drug Research

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Solvent concentration (1 mg/mL in MeOH)
Injection volume (1 μL)

dependent variables

LC-MS/MS analysis
Molecular networking

control variables

Previously published protocols (references 29 and 61)

controls

Positive control: Not specified
Negative control: Not specified

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