Characterization of Therapeutic Exosomes

cAd-MSCs and TK-cAd-MSCs exosomes were isolated by ultracentrifugation using 70 Ti rotor in an Optima LE-80K ultracentrifuge (Beckman Coulter) and characterized by transmission electron microscopy (TEM; Morgagni 268D electron microscope, Philips), Zetasizer Nano ZS (Malvern-Instruments), western-blot detection, and proteomic analysis by mass spectrometry were also carried out, as previously described [22 (link)]. Exosome quantification was performed using a Bicinchoninic acid kit (BCA; Thermo-Scientific).

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Villatoro A.J., Alcoholado C., Martín-Astorga M.D., Rubio N., Blanco J., Garrido C.P, & Becerra J. (2022). Suicide gene therapy by canine mesenchymal stem cell transduced with thymidine kinase in a u-87 glioblastoma murine model: Secretory profile and antitumor activity. PLoS ONE, 17(2), e0264001.

Publication 2022

Optima LE-80K Morgagni 268D electron microscope Zetasizer Nano ZS Bicinchoninic acid kit (BCA;

Bicinchoninic acid Electron microscope Exosome Mass spectrometry Transmission electron microscopy Ultracentrifugation Western blot

Corresponding Organization : Andalusian Centre for Nanomedicine and Biotechnology

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Variable analysis

independent variables

Exosomes from cAd-MSCs
Exosomes from TK-cAd-MSCs

dependent variables

Characterization of exosomes by transmission electron microscopy (TEM)
Characterization of exosomes by Zetasizer Nano ZS
Characterization of exosomes by western-blot detection
Characterization of exosomes by proteomic analysis by mass spectrometry
Exosome quantification using a Bicinchoninic acid kit (BCA)

control variables

Ultracentrifugation using 70 Ti rotor in an Optima LE-80K ultracentrifuge (Beckman Coulter)
Procedures for characterization of exosomes as previously described [22]

controls

No positive or negative controls were explicitly mentioned in the input.

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