6-Coumarin-loaded NPs were made dissolving 5 mg of 6-Coumarin (Sigma-Aldrich, Saint Louis, MO, USA) in 300 μl of a solution of 30 mg of PLGA-PEG-Bis-sulfone in THF. This solution was added dropwise to 7 ml of stirring water. The reaction was left to proceed for 3 h and then they were collected and centrifugated to be subsequently used for further analysis.
Protocol detail
Encapsulating 6-Coumarin in PLGA-PEG Nanoparticles
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Corresponding Organization :
Other organizations : Università Cattolica del Sacro Cuore, Istituto Pasteur, Sapienza University of Rome, Agostino Gemelli University Polyclinic, Istituti di Ricovero e Cura a Carattere Scientifico
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Variable analysis
independent variables
- Amount of 6-Coumarin (5 mg)
- Amount of PLGA-PEG-Bis-sulfone (30 mg)
- Volume of THF solvent (300 μl)
- Volume of water (7 ml)
- Duration of reaction (3 h)
- Properties of the 6-Coumarin-loaded NPs
- 6-Coumarin from Sigma-Aldrich, Saint Louis, MO, USA
- PLGA-PEG-Bis-sulfone used
- Stirring of the water during the reaction
- No positive or negative controls were explicitly mentioned in the provided information.
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