Human normal pancreatic duct epithelial cell HPDE6-C7, and pancreatic cancer cell lines PANC-1, SW1990, AsPC-1 and BxPC-3 were purchased from Shanghai Cell Bank (Shanghai, China). DMEM medium (11,965,084, Gibco, Waltham, MA, USA) containing 10% fetal bovine serum (FBS, 10,099,141, Gibco) and 1% penicillin-streptomycin (15,070,063, Gibco) was used to culture cells in a humidified atmosphere at 37°C with 5% CO2 [14 (link)].
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