According to the manufacturer’s instruction, morpholino antisense oligos (MOs; Gene Tools) were prepared at a stock concentration of 1 mM. We designed dusp14 splice-modifying morpholino (dusp14-MO) to knockdown the expression of dusp14 (Supplementary Table 3 ). MOs were diluted to 0.3 mM and injected into one-cell-stage embryos.
To generate the dusp14 gene mutant zebrafish, as described in our previous work (Gong et al., 2017 (link)), 2–3 nL of solution containing specific single-guide RNA (sgRNA) and Cas9 mRNA was injected into one-cell-stage embryos (primers used are listed inSupplementary Table 3 ).
To generate the dusp14 gene mutant zebrafish, as described in our previous work (Gong et al., 2017 (link)), 2–3 nL of solution containing specific single-guide RNA (sgRNA) and Cas9 mRNA was injected into one-cell-stage embryos (primers used are listed in
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