The drugs used were the following: AM404 [R&D Systems (Minneapolis, MN) and Fisher Scientific (Waltham, MA)]; MRS1477 and AM251 [Fisher Scientific]; capsazepine [VWR (Radnor, PA) and Cayman Chemical (Ann Arbor, MI)]; CP-55,940 [Santa Cruz Biotechnology (Dallas, TX)]; JZL184 [Sigma-Aldrich (St. Louis, MO) and Cayman Chemical]. All drugs were dissolved in 5% DMSO, 15% Tween 80 in sterile physiological saline and injected intraperitoneal at 10 mL/kg. The doses of drugs used were based on previous studies: AM404 at 10 mg/kg (Gamaleddin et al., 2013 (link)), MRS1477 at 10 mg/kg (Kaszas et al., 2012 (link)), AM251 at 1 mg/kg (Chen et al., 2004 (link)), capsazepine at 5 mg/kg (dos-Santos-Pereira et al., 2016 (link)), CP-55,940 at 30 μg/kg (Vinod et al., 2008 (link)), and JZL184 at two doses: 2 mg/kg and 18 mg/kg (Oleson et al., 2012 (link); Hartley et al., 2016 (link)).
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Pharmacological Modulation of Cannabinoid Receptors
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Am251
Am404
Capsazepine
Cayman
Cp 55 940
Dmso
Drugs
Jzl184
Mrs1477
Physiological
Saline
Sterile
Tween 80
Corresponding Organization : Yale University
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Variable analysis
independent variables
- AM404 (10 mg/kg)
- MRS1477 (10 mg/kg)
- AM251 (1 mg/kg)
- Capsazepine (5 mg/kg)
- CP-55,940 (30 μg/kg)
- JZL184 (2 mg/kg and 18 mg/kg)
- Not explicitly mentioned
- All drugs were dissolved in 5% DMSO, 15% Tween 80 in sterile physiological saline
- All drugs were injected intraperitoneally at 10 mL/kg
- Positive controls: Not specified
- Negative controls: Not specified
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