Murine Breast Cancer Cell Transfection

The murine breast cancer cell line 4 T1 (American Type Culture Collection, ATCC CRL-2539; Rockville, MD, USA) was transfected with a dsRed-expressing lentiviral vector (Discosoma sp. red fluorescent protein) [30 (link)]. Cells were cultured in RPMI-1640 medium (Bio-Whit-taker; Verviers, Belgium) containing fetal bovine serum (FBS;10%), Penicillin/Streptomycin (100 units/mL), non-essential amino acids (3.2%), plasmocin (0.005 mg/mL; InvitroGen; San Diego, CA, USA), and L-glutamine (400 mol/L; Lonza; Cologne, Germany), and were maintained in a humidified environment at 5% CO₂ and 37°C.

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Yang N., Huang B., Tsinkalovsky O., Brekkå N., Zhu H., Leiss L., Enger P.Ø., Li X, & Wang J. (2014). A novel GFP nude rat model to investigate tumor-stroma interactions. Cancer Cell International, 14, 541.

Publication 2014

RPMI-1640 medium Penicillin/Streptomycin plasmocin L-glutamine

Breast cancer cell line Cells Cultured medium Essential amino acids L glutamine Murine Penicillin Plasmocin Red fluorescent protein Streptomycin Vector

Corresponding Organization :

Other organizations : Qilu Hospital of Shandong University, University of Bergen, Shandong University

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Variable analysis

independent variables

Transfection of 4T1 murine breast cancer cells with a dsRed-expressing lentiviral vector

dependent variables

Expression of dsRed (Discosoma sp. red fluorescent protein) in 4T1 cells

control variables

Culture medium (RPMI-1640 containing 10% fetal bovine serum, Penicillin/Streptomycin, non-essential amino acids, plasmocin, and L-glutamine)
Incubation conditions (humidified environment at 5% CO2 and 37°C)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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