Novartis provided LCL161 (ref. 44 (link)). Tetralogic Pharmaceuticals provided Birinapant48 (link). AT-406, GDC-0917 and AZD-5582 were purchased from Active Biochem. TNF-α was purchased from Enzo. IFN-β was obtained from PBL Assay Science. Broad host range IFN-αB/D was produced in yeast and purified by affinity immunochromatography24 (link). Nontargeting siRNA or siRNA targeting cFLIP were obtained from Dharmacon (ON-TARGETplus SMARTpool). High molecular weight poly(I:C) was obtained from Invivogen.
AssayHost range Lcl161 Pharmaceuticals Poly i c Sirna Tnf α Yeast
Corresponding Organization :
Other organizations :
Children's Hospital of Eastern Ontario, University of Calgary, University Medical Center Freiburg, Moffitt Cancer Center, University of Ottawa
Birinapant (provided by Tetralogic Pharmaceuticals)
AT-406 (purchased from Active Biochem)
GDC-0917 (purchased from Active Biochem)
AZD-5582 (purchased from Active Biochem)
Nontargeting siRNA or siRNA targeting cFLIP (obtained from Dharmacon)
High molecular weight poly(I:C) (obtained from Invivogen)
dependent variables
Unspecified
control variables
TNF-α (purchased from Enzo)
IFN-β (obtained from PBL Assay Science)
Broad host range IFN-αB/D (produced in yeast and purified by affinity immunochromatography)
positive controls
Broad host range IFN-αB/D (produced in yeast and purified by affinity immunochromatography)
negative controls
Nontargeting siRNA
Annotations
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