The expression plasmids of GFP-FANCL harboring the C312,315A or C364,367A mutations were obtained from the plasmid for expressing the wild-type GFP-FANCL provided by Dr. Grover C. Bagby from Oregon Health and Science University17 (link) by using a GeneArt Site-directed Mutagenesis Kit (Thermo Fisher Scientific, Waltham, MA), following the vendor’s recommended procedures.
Protocol detail
Generating Site-Directed Mutants of GFP-FANCL
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Fancl
Mutations
Plasmid
Site directed mutagenesis
Corresponding Organization :
Other organizations : National Institute on Aging, National Institutes of Health
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Variable analysis
independent variables
- C312,315A mutation
- C364,367A mutation
- GFP-FANCL expression
- Plasmid for expressing the wild-type GFP-FANCL
- Positive control: Plasmid for expressing the wild-type GFP-FANCL
- Negative control: Not explicitly mentioned
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