Multilocus Sequence Typing of Enterobacter cloacae

Five E. cloacae strains the complete genome sequences of which have been determined (ATCC 13047, NCTC 9394, ENHKU 01, SCF1, and EcWSU 1; hereafter, genome strains) were used to design PCR primers. One hundred one clinical isolates collected at National Center for Global Health and Medicine Hospital and a commercial clinical laboratory (BML inc, Saitama, Japan) during 2007–2013 were used to evaluate the performance of the MLST scheme developed in the present study (Table 1).

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Miyoshi-Akiyama T., Hayakawa K., Ohmagari N., Shimojima M, & Kirikae T. (2013). Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae. PLoS ONE, 8(6), e66358.

Publication 2013

Clinical laboratory Cloacae Genome Medicine Primers Strains

Corresponding Organization :

Other organizations : National Center for Global Health and Medicine, Kawagoe Technical High School

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Variable analysis

independent variables

Five E. cloacae strains the complete genome sequences of which have been determined (ATCC 13047, NCTC 9394, ENHKU 01, SCF1, and EcWSU 1)

dependent variables

Performance of the MLST scheme developed in the present study

control variables

One hundred one clinical isolates collected at National Center for Global Health and Medicine Hospital and a commercial clinical laboratory (BML inc, Saitama, Japan) during 2007–2013

controls

Positive control: Not specified
Negative control: Not specified

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