Melanoma Cell Lines and Culture

M14 and A375 human melanoma cell lines were purchased from American Type Culture Collection (Manassas, VA). SBCL1 human melanoma cell line was provided by Bruno Giovanella^{53 (link)}. A375 human melanoma cells sensitive (A375S), resistant to Vemurafenib (A375R) or to Dabrafenib and Trametinib (A375DR), were previously characterized^{22 (link)}. HUVEC (PromoCell GmbH, Heidelberg, Germany) were cultured in complete EBM-2 medium (Clonetics Bio Whittaker, now Lonza, Cologne, Germany), containing 2% fetal bovine serum (FBS). All cell lines were grown in RPMI medium (Euroclone, Milan, IT) supplemented with 10% (v/v) FBS, 1% penicillin/streptomycin and 1% L-glutamine (Euroclone) at 37 °C in a balanced air humidified incubator with 5% CO_2. They have been routinely tested for mycoplasma contamination and were recently authenticated (STR profiling) and tested for mycoplasma contamination.