Expression Plasmid Generation for Def6, Raptor, and p62

Expression plasmids for untagged and HA-tagged Def6 were generated as described previously^{21 (link)}. Expression constructs for Myc-tagged raptor (Addgene plasmid 1859^{53 (link)}) and HA-tagged p62 (Addgene plasmid 28027^{54 (link)}) were purchased from Addgene. Expression plasmids for Flag-tagged TRAF6 and Flag-tagged full-length p62 and its various deletion mutants were constructed in p3XFLAG-CMV-10 expression vector (Sigma) and transfected into 293T (CRL-3216; ATCC) cells.

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Yi W., Gupta S., Ricker E., Manni M., Jessberger R., Chinenov Y., Molina H, & Pernis A.B. (2017). The mTORC1-4E-BP-eIF4E axis controls de novo Bcl6 protein synthesis in T cells and systemic autoimmunity. Nature Communications, 8, 254.

Publication 2017

p3XFLAG-CMV-10 expression vector

Cells Deletion Plasmids Raptor Traf6 Vector

Corresponding Organization :

Other organizations : Hospital for Special Surgery, Cornell University, TU Dresden, Rockefeller University

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Variable analysis

independent variables

Expression plasmids for untagged and HA-tagged Def6
Expression constructs for Myc-tagged raptor
Expression plasmids for HA-tagged p62
Expression plasmids for Flag-tagged TRAF6
Expression plasmids for Flag-tagged full-length p62 and its various deletion mutants

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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