Real-time PCR was used to measure the expression of p21, 14–3-3 (YWHAQ), GADD45, PUMA (BBC3), Bax, MDM2, Noxa (PMAIP1), CXCR4, and NMYC. GAPDH, EEF1A1, and TBP were used as a normalization control (Additional file 2 : Table S1). PrimeScript Reverse Transcriptase enzyme (Takara Bio Inc., Japan) and 2x Power SYBR Green Master Mix (Applied Biosystems, USA) was used for generation of cDNA and real-time PCR according to the manufacturer’s recommendations. PCR was performed on an SDS 7900HT instrument (Applied Biosystems, USA). Raw Ct values obtained with SDS 2.2 (Applied Biosystems, USA) were imported in Excel and normalization factor and fold changes were calculated using the GeNorm method [30 (link)].
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